| Windsor Regional | Oshawa Regional | ||||
| Region | School | Seed | Region | School | Seed |
| TDCAA | Bishop Allen | 1 | LOSSA | Sinclair | 1 |
| SWOSSAA | St. Anne | 2 | NOSSA | St. Charles College | 2 |
| CISAA | Upper Canada College | 3 | LOSSA | Donald A Wilson | 3 |
| TDSSAA | York Mills | 4 | COSSA | St. Peter’s | 4 |
| SWOSSAA | Riverside | 5 | ROPSSAA | Mount Carmel | 5 |
| GHAC | Milton | 6 | GBSSA | Collingwood CI | 6 |
| CWOSSA | Sacred Heart | 7 | YRAA | St. Theresa of Lisieux | 7 |
| SOSSA | West Niagara | 8 | NCSSAA | Sir Robert Borden | 8 |
| WOSSAA | Glendale | 9 | EOSSAA | Thousand Islands | 9 |