| ASSOCIATION | SCHOOL | SEED |
| CISAA | St. Michael’s College School | 4 |
| CISAA | Upper Canada College | 14 |
| COSSA | Eastside Secondary School | 17 |
| CWOSSAA | St. Mary’s High School Kitchener | 12 |
| EOSSAA | Frontenac Secondary School | 8 |
| GBSSA | Bradford District High School | 18 |
| GHAC | St. John Henry Newman Catholic Secondary School | 10 |
| LOSSA | Pickering High School | 9 |
| NCSSAA | St. Patrick’s High School | 15 |
| NOSSA | Lo-Ellen Park Secondary School | 13 |
| ROPSSAA | Notre Dame Catholic Secondary School | 3 |
| SOSSA | Eden High School | 7 |
| SWOSSAA | Ursuline College | 2 |
| SWOSSAA | Sarnia Northern Collegiate Institute and Vocational School | 5 |
| TDCAA | St. Mother Teresa Catholic Academy | 6 |
| TDSSAA | Oakwood Collegiate Institute | 1 |
| WOSSAA | Sir Frederick Banting Secondary School | 11 |
| YRAA | Thornlea Secondary School | 16 |