| Association | School | Seed |
| HOST – CWOSSA | Walkerton District Community School (Walkerton) | 10 |
| COSSA | Nicholson Catholic College (Belleville) | 2 |
| CWOSSA | Woodland Christian High School (Breslau) | 3 |
| EOSSAA | St. Mary Catholic High School (Brockville) | 4 |
| GBSSA | E.S.C. Nouvelle-Alliance (Barrie) | 16 |
| GHAC | ESC Ste-Trinité (Oakville) | 11 |
| NCSSA | Osgoode Township High School (Ottawa) | 7 |
| NEOAA | Roland Michener Secondary School (South Porcupine) | 5 |
| NOSSA | E.S.C. L’Horizon (Sudbury) | 13 |
| NWOSSA | St. Thomas Aquinas High School (Kenora) | 15 |
| SOSSA | Hamilton District Christian High School (Ancaster) | 1 |
| SOSSA | Smithville Christian High School (Smithville) | 6 |
| SWOSSAA | ESC E.J. Lajeunesse (Windsor) | 12 |
| TDCAA | University of Toronto Schools (Toronto) | 14 |
| TDSSAA | Oakwood Collegiate Institute (Toronto) | 9 |
| WOSSAA | F.E. Madill (Wingham) | 8 |